Description
Pre-made Lentivirus express human target: TERT (telomerase reverse transcriptase), (alternative name: EST2, TCS1, TP2, TRT, hEST2), 100% matched CDS region in NCBI ID: NM_198253.
The hTERT is constantly expressed under an optional inducible CMV promoter (It can be turned into inducible expression when its TetR repressor is present). A Blasticidin-RFP fusion marker, under RSV promoter, allows to sort or select transduced cells via RFP signal or via blasticidin antibiotic (Dual markers). RFP signal provides convenient, real-time monitoring the performance of the particle. [Note: see also the GFP–Puromycin version of this product, CAT#: LVP105-GP. ].
Telomerase expression plays a role in cellular senescence. Over-expression of hTERT was found to immortalize certain cell types as well as impart different interesting properties to different stem cells.
See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium, premixed with Polybrene. (see also its concentrated lentivirus, CAT#: LVP105-PBS)
Cat#: LVP105
