Description
Pre-made optional inducible lentiviral particles for expressing human target: TRPV1 (alternative name: VR1, OTRPC1). The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID: NM_080704.3 . Particles also contains a RFP-Blasticidin dual selection marker.
Full sequence verified target is natively expressed under a tetracycline inducible suCMV promoter (Note: it can also used as regular constitutive high expression without any inducation required). A RFP-Blasticidin fusion marker under RSV promoter, allows to sort or select transduced cells for long term expression via RFP signal or via blasticidin antibiotic (Dual markers). RFP signal provides a convenient, realtime monitoring the particles performance.
Each particles was validated in lot by lot basis and target expression is guaranteed. Particles were packaged in serum-free solution containing no any human or animal origin components. See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium (with 10x polybrene).
Cat#: LVP503