Description
Premade Lentivirus expressing human target: thyroid stimulating hormone receptor (TSHR) (alternative name: CHNG1, LGR3, hTSHR-I). The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID: NM_000369. It constitutively expresses the target without need for any induction.
The target was expressed under the optional inducible CMV promoter (TetCMV) (click to see more technical details). Yet, optionally, It can be used for the inducible expression when used together with its repressor (TetR). For generating inducible expression cell line, simply apply this target expression lentivirus into TetR ready Cell lines.
It has the RFP-Blasticidin fusion marker under RSV promoter, allows to sort or select transduced cells for long term expression via RFP signal or via blasticidin antibiotic (Dual markers). RFP signal provides a convenient, realtime monitoring the particles performance. . See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium with 10x Polybrene.
Cat#: LVP510
