Human RPMI 8226 (Luciferase & GFP) Stable Cells

$2,500.00

Human RPMI 8226 (Luciferase & GFP) Dual Reporter Cell line

SKU: SC060-LG Categories: ,

Description

The human RPMI 8226 cell line ( a B Lymphocyte with surface antigen profile: HLA Aw19, B15, B37, Cw2; CD19 -; CD20 -; CD28 +; CD38 +; CD49e +) was originated from a human peripheral blood with multiple myeloma from 61 years male.

This dual reporter cell line has the Luciferase and GFP constitutively co-expressed under EF1a promoter as two separate protein (not as fusion), mediated via 2A element. The Puromycin resistance marker was expressed under Rsv promoter. The following express cassette was integrated into cell line’s genome. 

GFP and Luciferase co-expression under EF1a promoter

Each cell demonstrates strong GFP fluorescent signal and luciferase assay signal via Luc assay (Note: It is the pooled stable cell population, not originated from a single cell colony. Thus you will observe the uneven GFP signal intensity amount cells).

Sold at:  1 vial x (2 x 106 cells)/vial, see details in Product Manual.

Cat# SC060-LG