Description
The VP64 domain is derived from the herpes simplex virus VP16 protein and contains four copies of a 64-amino-acid sequence that can recruit various transcriptional co-activators to enhance gene transcription [Reference]. The SunTag is multiple copies of SGSG peptide tag, separated by a flexible linker (GGGGG). SunTag allows for multiple copies of an activation domain to be recruited to the target site for efficient gene activation [Reference]. The “dCas9-VP64-SunTag” fusion creates a CRISPR based, highly potent transcriptional activator for the targeted genes (CRISPRa).
This premade lentivirus express the dCa9-VP64-SunTag fusion under the enhanced CMV promoter (SuCMV), carrying Neomycin (G418) selection. It can be used to activate the desired genes, or generate a genome-wide activation-ready cell line, which can then be used for the targeted gene-expression activation upon gRNA addition for screening and profiling assay.
If desired, the negative control lentivirus, CAT#: LVP1596, which has the dCas9 fusioned with a Null sequence, can be used as the non-specific, background control.
To up-regulate your desired gene, simply apply this lentivirus to your cells and then add the targeting gRNA lentivirus (which need to be designed and made separately according to your target locus.), or apply this lentivirus together with your desired gRNA lentivirus. (Note: Contact us if you need to make your desired gRNA lentivirus via a customized order).
see details in Product Manual.
Amount: 200 ul/vial, at titer of 1×108 IFU/ml, provided in PBS solution (premixed with 10x polybrene, 60 ug/ml).
CAT#: LVP1572
