Description
This premade Lentivirus expresses human GNAS target, (Gene_Name: GNAS complex locus ), with coding sequence 100% matching to CDS region in NCBI ID: NM_000516 (gene_synonym: AHO; C20orf45; GNAS1; GPSA; GSA; GSP; NESP; PITA3; POH; SCG6; SgVI).
The full sequence-verified target is expressed under the enhanced EF1a promoter (which is best suite for the hard-to-transduced cell types and suspension cells, like T Cells, B Cells). It also contains a GFP-Puromycin dual selection marker (see lentivector scheme above). The GFP signal provides convenient, real-time monitoring of the particles’ performance. See Product Manual for details.
When desired, use the cognate Null-control Lentivirus (CAT#: EF1a-Null-GP-PBS) as the negative control, for lentivirus treatment in your cell culture. The Null-Control does not express any targets, but express the same GFP-Puromycin selection marker.
Amount: 200ul, at 1 x 108 IFU/ml (Premixed with 10x Polybrene / 60ug/ml)
Cat#: LVP1698
