Description
Color–Switch, Flp reporter stable cell line:
This cell line was transformed from HEK293 Cell line, expressing “FRT-GFP-stop-FRT-RFP” cassette under CMV promoter (see Color-Switch cassette scheme below). Cell line also contains a Puromycin antibiotic marker under RSV promoter (not shown in the scheme).
Applications:
- This Flp reporting cell lines is used to monitor the efficiency of Flp recombination in vivo. It is a great method and easy tool to verify the recombination event by Flp enzyme.
- It is a convenient tool or controls, to verify your Flp-FRTbased system.
- It can also used to verify your CRISPR editing efficiency by targeting at FRTs and GFP/RFP region (please be advised, we do not provide any sequence information unless a license is purchased for this cell line).
How it works?
The cell line demonstrates strong GFP (Green Fluorescent signal). The downstream RFP (Red Fluorescent Protein) was not expressed because of the stop codon after the GFP. Once the Flp enzyme is present in nuclear, the Flp excises / deletes the DNA fragment between two loxP sites, which removes the “GFP-stop codon” section (see the DNA structure scheme above). As a result, the RFP is then expressed, and the cell line switches to RFP fluorescent. The RFP signal can be easily visualized under Fluorescent microscope, or measured for the fluorescent intensity if desired.
Sample image of Flp-FRT recombination detection:
Please click here for Product Manual.
Sold at: 1 vial x (2 x 106 cells)/vial
Cat#: SC096-Puro
