RFP (EF1a, Puro), Concentrated lentivirus

Lentivirus expresses codon optimized Red Fluorescent Protein (RFP) under an enhanced EF1a promoter. A Puromycin (Puro) selection marker was express under Rsv promoter, which allows to select transduced fluorescent positive cells. See Product Manual (.pdf).

The re-engineered EF1a promoter is strong constitutive promoter. It is non-tissue specific, highly expressed in all cell types, and less likely be silenced after long-term culture. 

Application:

1. monitor lentivirus transduction efficiency for your cells of interest;
2. stable cell line generation;
3. others;