Other Immortalization Factors
The most widely use cell immortalization methods are to over-express SV40 large T-antigen or human TERT gene. In addition Sv40-T-antigen and hTERT gene, the other genes are also used for cell immortalization depends on cell types. The Epstein Barr Virus (EBV) genes (EBNA1 and EBNA2) were reported used for immortalize B and T lymphocytes, the HPV16 virus’ E6/E7 genes for keratinocytes, the Adenovirus type 5’s E1A gene for primary rodent cells, the human HOX genes for various hematopoietic cells, including macrophages, hematopoietic progenitor cells, and myeloid progenitor cells, the human CDK4 for human bronchial cells and myogenic cells, the human KRas V12 mutant, cMyc for a wide variety of cells and so on.
Gentarget provides the premade over-expression lentivirus products for those cell immortalization genes. Each gene was expressed under an enhanced enhanced EF1a promoter which is active in almost all cell types and less likely to be silenced during long-term culture. Each Lentivirus is featured with an antibiotic-fluorescent fusion dual maker, GFP–puromycin or RFP–Blasticidin. (see vector map scheme below).
Lentivirus are provided as in two formats:
- Crude Lentivirus: 200ul /vial in DMEM containing 10 x Polybrene;
- Concentrated lentivirus: 200ul PBS solution which is better for hard-to-transduced cell types or for serum-free culture.
Please click each product below, or see Product Manual for details.