P53 siRNA Lentivirus
P53 siRNA for Cell Immortalization:
Knockdown or repress human “tumor Suppressor Proteins”, like P53 (NM_000546) can immortalize a wide variety of cell types.
GenTarget offers the shRNA Lentivirus that knockdown human P53 gene. These pre-made knockdown lentivirus are validated with greater than 75% knockdown level (80% to 97% depends upon cell types). Each P53 shRNA lentivirus contains a Fluorescent-Antibiotic fusion dual marker so you can either select the transduced cells by antibiotic killing or sort them via Flow Cytometer. Those products are available in four different dual markers versions as: GFP-Blasticidin, GFP-Puromycin, RFP-Blasticidin and RFP-Puromycin. See shRNA lentivector core structure scheme below.
Lentivirus are provided as in two formats:
- Crude Lentivirus: 200ul /vial in DMEM containing 10 x Polybrene;
- Concentrated lentivirus: 200ul PBS solution which is better for hard-to-transduced cell types or for serum-free culture.
Please see Product Manual for details.