B-Cell Reporter
About B29 Promoter:
The promoter of B-cell-specific B29 gene (CD79b) exhibits B-cell-specific activity. It lacks either a TATA or a CAAT box and its transcription is initiated at multiple sites.
Utilizing C79b’s promoter, GenTarget developed a set of reporting lentivirus for specifically or preferably labeling B-Cells. Those reporting lentivirus has a luminescent report or a fluorescent report under the promoter of human B29 gene (click to see its sequence) that specific expressed in B cells.
Those reporter lentivirus are best suitable for infecting the human or mouse B-cells, as well as for the signal pathway research on B29 promoter regulation. Those reporter lentivirus provide a much easier tool to specifically labeling or reporting for human or mouse B-Cell in vitro and in vivo via the luciferase signal or fluorescent signal.
Those reporting lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. This selection marker is used to select the lentivirus infected cells (to generate the stable cell lines) via antibiotic killing or fluorescent cell sorting. See the scheme below for lentivector core expression cassette.
see Product Manual for details (.pdf).