Endothelial Cell Report
About FLT1 Promoter:
Human FLT1 gene encodes Vascular endothelial growth factor receptor 1 (VEGFR1) which is one of the receptors for VEGF. It contains an extracellular ligand-binding region, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain.
Flt-1 is expressed specifically in endothelial cells and up-regulated in tumor vasculature. Its promoter region contains a TATA box, a GC-rich region, nine Ets motifs and one CREB/ATF motif.
Label / Imaging Endothelial Cells:
GenTarget developed a set of Report lentivirus specifically label Endothelial Cells. Those reporting lentivirus has a luminescent report or a fluorescent report under the promoter of human FLT1 gene (click to see its sequence) that specific expressed in Endothelial Cells.
Those reporting lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. This selection marker is used to select the lentivirus infected cells (to generate the stable cell lines) via antibiotic killing or fluorescent cell sorting. See the scheme below for lentivector core expression cassette.
The premade, ready-to-use reporter lentivirus provides a much easier tool to specifically labeling or live cell imaging of human or mouse Endothelial Cells in vitro and in vivo via the luciferase signal or fluorescent signal.
see Product Manual for details (.pdf).