Kidney Cell Report
About Nephrin Promoter:
Nephrin expression is restricted to kidney podocytes. Nephrin promoter is kidney’s glomerular-specific promoter that capable of directing podocyte-specific expression.
Report Lentivirus for Kidney Cells:
GenTarget developed a set of reporting lentivirus for selectively labeling kidney podocytes cells. Those reporting lentivirus has a luminescent report or a fluorescent report under the promoter of human Nephrin gene (click to see its sequence) that specifically expressed in kidney glomerular cells. Those reporter lentivirus are best suitable for infecting the human or mouse Kidney cell lines, as well as for the signal pathway research on Nephrin promoter regulation. Down-regulation of nephrin in podocytes leads to development of kidney diseases. Those reporter lentivirus provide a convenient tool for screening and identifying the pathophysiologic substances that regulate nephrin expression.
Those reporting lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. This selection marker is used to select the lentivirus infected cells (to generate the stable cell lines) via antibiotic killing or fluorescent cell sorting. See the scheme below for lentivector core expression cassette.
The premade, ready-to-use reporter lentivirus provides a much easier tool to specifically labeling or reporting for human or mouse kidney cells in vitro and in vivo via the luciferase signal or fluorescent signal.
see Product Manual for details (.pdf).