Lung Tissue Report

About SPB  Promoter:

Surfactant protein B (SPB) is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. It is a developmentally and hormonally regulated lung protein. The human SPB promoter contains two cis-acting elements for transcription factors that involved in the specificity and the activation of gene expression in the lung.

Report Lentivirus for Lung Tissue:

GenTarget developed a set of reporting lentivirus for selectively labeling bronchiolar and alveolar epithelial cells of the lung. Those reporting lentivirus has a luminescent report or a fluorescent report under the promoter of human SPB  gene (click to see its sequence) that specifically expressed in bronchiolar and alveolar epithelial cells of the lung. Those reporter lentivirus are best suitable for infecting the human or mouse Lung Tissue / Cells, as well as for the signal pathway research on SPB  promoter regulation.

Those reporting lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. This selection marker is used to select the lentivirus infected cells (to generate the stable cell lines) via antibiotic killing or fluorescent cell sorting. See the scheme below for lentivector core expression cassette.

The premade, ready-to-use reporter lentivirus provides a much easier tool to specifically labeling or reporting for human or mouse Lung Tissue / Cells in vitro and in vivo via the luciferase signal or fluorescent signal.

see Product Manual for details (.pdf).