Macrophages Report
About CD68 Promoter:
CD68 (Cluster of Differentiation 68) is a glycosylated transmembrane proteins expressed specifically in monocytes / macrophages and macrophage-related cells. It is particularly useful as a marker for the various cells of the macrophage lineage. The CD68 gene promoter directs macrophage-specific expression. The promoter contains transcriptional element with preferential monocyte / macrophage gene expression.
Report Lentivirus for Macrophases:
GenTarget developed a set of reporting lentivirus for specifically labeling Macrophages. Those reporting lentivirus has a luminescent report or a fluorescent report under the promoter of human CD68 gene (click to see its sequence) that specific expressed in Macrophages. Those reporter lentivirus are best suitable for infecting the human or mouse in both monocyte and embryo-derived tissue resident macrophages, as well as for the signal pathway research on CD68 promoter regulation.
Those reporting lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. This selection marker is used to select the lentivirus infected cells (to generate the stable cell lines) via antibiotic killing or fluorescent cell sorting. See the scheme below for lentivector core expression cassette.
The premade, ready-to-use reporter lentivirus provides a much easier tool to specifically labeling or reporting for human or mouse Macrophages for analyzing monocyte mobilization and monocyte trafficking in vitro and in vivo via the luciferase signal or fluorescent signal.
see Product Manual for details (.pdf).