Megakaryocytes Report
About ICAM2 (CD102) Promoter:
Intercellular adhesion molecule 2 (ICAM2), also known as CD102, is a cell surface adhesion molecule constitutively expressed in vascular endothelial cells and megakaryocytes, involved in leukocyte recruitment into tissues. Its promoter contains four transcription factor binding sites such as Sp1 and GATA motifs but no TATA box. Human ICAM-2 promoter contains all of the signals necessary for endothelium-specific transgene expression. The promoter activity is down-regulated by TNF-α. Reporter signal driven by this promoter was 125-fold greater in bovine aortic endothelial cells but less than 2-fold in non-endothelial (COS) cells, contrast to background.
Lentivirus Labels Megakaryocytes:
GenTarget developed a set of reporting lentivirus for specifically labeling vasculature endothelial cells and megakaryocytes. Those reporting lentivirus has a luminescent report or a fluorescent report under the promoter of human ICAM2 gene (click to see its sequence) that constitutively expressed in vasculature endothelial cells and megakaryocytes. Those reporter lentivirus are best suitable for infecting the human or mouse vasculature endothelial cells and megakaryocytes, as well as for the signal pathway research on ICAM2 promoter regulation.
Those reporting lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. This selection marker is used to select the lentivirus infected cells (to generate the stable cell lines) via antibiotic killing or fluorescent cell sorting. See the scheme below for lentivector core expression cassette.
The premade, ready-to-use reporter lentivirus provides a much easier tool to specifically labeling or reporting for human or mouse vasculature endothelial cells and megakaryocytes in vitro and in vivo via the luciferase signal or fluorescent signal.
see Product Manual for details (.pdf).