Pancreatic Cancer Report
About CCKAR Promoter:
The human Cholecystokinin A receptor (CCKAR) is a major physiologic mediator of pancreatic enzyme secretion and is predominantly expressed in human pancreatic cancer. The promoter of CCKAR has been shown as a strong pancreatic cancer-specific promoter. This promoter lacks a typical TATA box but contains two GC-box motifs which directs the pancreatic cancer-specific expression.
Label Pancreatic Cancer:
GenTarget developed a set of reporting lentivirus for selectively labeling pancreatic cancer cells. Those reporting lentivirus has a luminescent report or a fluorescent report under the promoter of human CCKAR gene (click to see its sequence) that specifically expressed in pancreatic cancer cells. Those reporter lentivirus are best suitable for infecting the human or mouse pancreatic cancer cells, as well as for the signal pathway research on CCKAR promoter regulation.
Those reporting lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. This selection marker is used to select the lentivirus infected cells (to generate the stable cell lines) via antibiotic killing or fluorescent cell sorting. See the scheme below for lentivector core expression cassette.
The premade, ready-to-use reporter lentivirus provides a much easier tool to specifically labeling or reporting for human or mouse pancreatic cancer cells in vitro and in vivo via the luciferase signal or fluorescent signal.
see Product Manual for details (.pdf).