The Best and Easiest PCR Cloning method

What is Eco-Cloning?

GenTarget’s revolutionary proprietary Fusion in vivo high throughput Eco cloning technology (patent pending) is the easiest PCR cloning method available for making E Coli expression clones. It utilizes an engineered E Coli strain with enhanced homologous recombination machinery for cloning PCR products. A specific vector is already built into the cloning cells for an in vivo end-joining reaction. A proprietary processing technology ensures low background and a > 95% positive cloning rate.

How it works?

Simply amplify your gene of interest with a primer pair that is flanked with a short arm homologous to the expression vector, then add 1µl of purified PCR into the engineered, ready-to-use cloning cells, and immediately proceed to transformed. There is no need to purchase either the expression vectors or competent cells. The optimized E Coli or mammalian expression vector is already built into the cloning cells. You just select the cloning cells having the built-in vector that meet your needs.

This extremely easy PCR cloning method also eliminates the labor-intensive and sometimes troublesome vector preparation procedures of other cloning technologies. No in vitro tube reaction is required (such as ligation, Topo TA cloning, or In-fusion reaction). Let  E Coli do the job for you in vivo! 

Key Features: 

• No need to buy vectors, thus eliminates the tedious bench work of preparing vector backbone;
• No need to buy cloning competent cells
• No need for any enzymes or any tube reactions
• Precise directional cloning of PCR products without any extra amino acids except the affinity tag
• Flexibility to allow addition of any cleavage site for removal of N-term His-tag if desired
• Works with any PCR products with or without a 3′-end’s -A overhung (the extra -A overhang, if exists, will be removed in cloning step)
• Good for different PCR sizes, from 200bp to 10kb and the same PCR product can be used to construct multiple different expression vectors
• Engineered E Coli and mammalian expression vectors for high protein yields
• Great for high through-put cloning
• The easiest and most cost-effective PCR cloning method available

How to select PCR cloning kit:

Note: For E Coli expression, the protein is expressed from either a T7 based or non-T7 based vector, depending on the cloning kit.  For expressing toxic proteins, please select our basal level self-controlled PCR cloning cells (Cat: IC-1003).