Assay Cell Lines
Premade cell lines generated for different categories listed below,
- A pathway specific target is constitutively expressed under an enhance EF1a promoter or enhanced CMV promoter;
- Fluorescent reporter or Luciferase reporter is expressed under an enhance EF1a promoter or enhanced CMV promoter;
- Cell line express a specific cell receptor.
One of the following expression cassette is integrated into each cell line:
For example, to evaluate the targeted killing efficacy of CAR-T, Gentarget generates many targeted cell lines from different tumor cell types that are labeled with a fluorescent (GFP or RFP), or a tumor target (for example, CD19, PDL1 cell surface marker). When the target cells are co-cultured with the CAR engineered T cells, the target cells are killed and lost the fluorescent signal as results of cell lysis. The live cells can be imaged and monitored at real-time to measure the tumor cell lysis in Flow cytometry. It provides a fast, easy validation approach for CAR-T products. Those cell lines provide convenient, real-time monitoring of CAR efficacy and other pathway-specific reactions, in dose and time course matter.
Please click each product’s link below for product details.