miRNA expression Lentivirus:

What is microRNA (miRNA)?

A microRNA (miRNA) is a small non-coding RNA molecule (containing about 22 nucleotides) found in plants, animals and some viruses. Each miRNA regulate multiple gene functions in RNA silencing and post-transcriptional regulation. Over-expression microRNA is a tool for investigation of  group gene’s regulation and function.

Gentarget’s microRNA expression lentivector:

Human or mouse microRNA precursors with its native context sequences (upstream and downstream flanking genomic sequences) were PCR amplified and cloned into a pLenti-TetCMV(GFP-Stop-3UTR/miRNA)-Rsv (Puro) lentivector. The GFP and pre-miRNA are co-transcribed under the same promoter, the enhanced CMV promoter. GFP provides a convenient indicator for miRNA expression levels, and a Puromycin antibiotic selection marker (under a Rsv promoter) provides the selection method for long term stable expression. See the scheme below for the core vector structure.

Gentarget provides two sub-categories of microRNA expression:

1.  Premade microRNA expression lentivirus:

Those are premade, in stock, ready to ship. Please search your microRNA ID for the product. Each order contains:

1. 1. 1. 0.2 ml of specific miRNA lentivirus
      2. 0.2 ml of negative control miRNA lentivirus

2. Made-on-Demand microRNA expression lentivirus set:

If you do not find target miRNA, we can make any miRNAs that are listed in the miRBase database. The timeline is 2 weeks. You can place a custom order here, or request a service quote by Email.  Each order contains:

1) 0.5 ml of specific miRNA lentivirus

2) 0.5 ml of negative control miRNA lentivirus

( See Product Manual for details. )

Click to see: Human miRNA expression lentivirus product list.

Click to see: Mouse miRNA expression lentivirus product list.