About Inducing Pluripotent Stem Cells (iPSC):

Conversion of  fully differentiated mouse or human somatic cells into embryonic-like cells (so called induced Pluripotent Stem Cells or iPSCs) has attracted enormous attention. Multiple reports have demonstrated that iPS cells can be generated by using a set of transcription factors or stem cell factors delivered via expression virus or by expressed proteins. The main stem cell factors are: OCT3/4, SOX2, NANOG, LIN28, c-Myc, and KLF4, although the combination of reprogramming factors may be slightly different. iPSCs hold the promise of curing many human diseases and accelerating stem cell research. 

Lentivirus express iPS factors:

Gentarget Provides the iPSC expression lentivirus driven by either the optional super CMV promoter or EF1α promoters, for all six human and mouse stem cell factors.

1. iPS Lentiviraus under CMV Promoter:

Those lentivirus contains either the RFP-Blasticidin or Neomycin antibiotic marker (see vector scheme below). 

These lentiviruses can be used for constitutive high expression and can also be used for tetracycline-inducible expression when a repressor regulator protein (TetR) is present.(see How etracycline-inducible expression works).

2. iPS Lentiviraus under CMV Promoter:

Those lentivirus contains either the RFP-Puromycin or Puromycin antibiotic marker (see vector scheme below). 

3. Four iPS factor chained Lentivirus:

Those Lentivirus express the four chained factors in a single lentivector, see its scheme below:

 

iPS lentiviral particles provide an easy tool to generate your own iPS cells. Gentarget’s iPS lentivirus was validated in generation of iPS cell and you can get your iPS cells in as short as in 14 days. Please refer to our validated iPS cell generation procedures. 

Gentarget’s  iPS Lentivirus includes: 

A) Human iPS factors (NANOG, OCT4, SOX2, KLF4, Myc, LIN28).

B) Mouse iPS factors (NANOG, OCT4, SOX2, KLF4, Myc, LIN28). 

C) Other potential iPS factors.

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iPS clone images generated using Gentarget’s iPS lentiviral particles:

[ Images above were provided by Dr. G. Wang in Harvard Medical School, Boston, MA 02115.]

References:

1. Masaki Ieda, Ji-Dong Fu, et al. (2010). Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes by Defined Factors. Cell 142, 375-386.
2. Takahashi, K. and Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676.
3. Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007). Induced pluripotent stem cell lines derived from human somatic cells. Science 318, 1917-1920.
4. Park, I.H., et al., Reprogramming of human somatic cells to pluripotency with defined factors. Nature, 2008. 451(7175): p. 141-6.
5. Shao, L., et al., Generation of iPS cells using defined factors linked via the self-cleaving 2A sequences in a single open reading frame. Cell Res., 2009. 19(3): p. 296-306.