CRE and Flp reporting cells

 1. About CRE-LoxP for GFP/RFP color switch:

Those reporting cell lines, derived from HEK293 cells, constitutively expresses a “LoxP-GFP-Stop-LoxP-RFP-Stop” cassette under strong suCMV promoter. See the genomic integrated express cassette structure below.

When CRE enzyme is present, CRE-LoxP recombination will excise out the GFP region between two loxP sites, resulting GFP signal disappearing and RFP signal being turned on in the cells.  

 2. About Flp-FRT for GFP/RFP color switch:

Those reporting cell lings express the “FRT-GFP-stop-FRT-RFP-Stop” cassette under either an enhanced CMV promoter or EF1a promoter. See the genomic integrated express cassette structure below.

When FLP protein is present in the nucleus, the FLP excises / deletes the DNA fragment between two FRT sites.  As a result, the GFP is removed and RFP is expressed, resulted in high RFP expression.

Gentarget developed the reporting Cell lines containing different antibiotic selection maker, Puromycin, Neomycin or Blasticidin. see details in CRE Reporting cell lines Manual  or Flp /FRT Reporting Cell line Manual.

Product Citations:

1. ADIPOR1 is essential for vision and its RPE expression is lost in the Mfrp rd6 mouse;
2. In vivo screening identifies GATAD2B as a metastasis driver in KRAS-driven lung cancer;