Knockdown Validated shRNA Lentivirus:

GenTarget’s premade Lentiviral Particles are the most advanced tools available for delivering target over-expression or knockdown into a wide range of cell types including primary and non-dividing cells. The pre-made shRNA lentivirus demonstrates greater than 75~95% knockdown levels validated in in a reporter assay where the specific target was fusioned with lacZ or luciferase reporter. The knockdown levels were reflected by decreases of lacZ or luciferase activity. 

Applications: 

1. 1. Delivery of  expression knockdown into hard-to-transfect cell types (such as neuronal cells) without the use of transfection reagents
   2. Creation of knockdown stable cell lines for long-term study
   3. Knockdown of target in primary or drug-arrested cells 

Product Description: 

Each shRNA lentiviral particles (or lentivirus) is guaranteed to knockdown the specified endogenous target. The H1 driven shRNA lentivirus can be used for constitutive, or optionally for tetracycline- inducible knockdown. The U6 driven shRNA are used for constitutive knockdown. They contain an antibiotic marker or a “fluorescent-antibiotic” fusion dual marker (GFP or RFP fusion with puromycin or blasticidin) for both antibiotic selection and/or fluorescence sorting. See the shRNA core map scheme below.

Questions?  Please see FAQs for pre-made lentiviral particles. 

Please click  here for Transduction protocols for adhesive and suspension cells. 

Features of validated shRNA lentivirus: 

1. 1. Easy-to-use: directly add particles to cells. There is no need for any lipids or transfection reagents
   2. 75% to 95% knockdown, depending on cell types
   3. Optional inducible shRNA expression: Particles can be used for constitutive knockdown or for Tet- inducible shRNA expression in the presence of TetR (for H1 promoter driven shRNA only).
   4. Safety: Self-inactivation prevents replication of the virion
   5. High titer ready to use:  provided at 1 x 10⁸ IFU/ml titer, in PBS solution premixed with 10x Polybrene (60ug/ml);
   6. Dual selection markers: easy real-time monitoring of target expression by fluorescence and antibiotic selection of positive transduced cells with puromycin or blasticidin  

Custom shRNA: 

Not find the shRNA for your gene? No problems. We also provide Custom shRNA Services to generate your specific shRNA lentiviral particles. Please contact us for a service quote!