c-Myc Pathway

1. About c-Myc Pathway:

c-Myc is a transcription factor and a member of the Myc family of regulator genes and proto-oncogenes. c-Myc functions as a transcription factor by binding to E-box sequences in the promoter regions of its target genes, regulating their expression that involved in various cellular processes. c-Myc is often upregulated in tumors and contributes to around 40% of cancers. The c-Myc signaling pathway provides critical insights into cellular regulation and cancer biology, making it a significant focus for research and therapeutic development  [Ref1].

2. Lentivirus Detects c-Myc Pathway:

GenTarget developed a set of reporting lentivirus to monitor c-Myc  signaling pathway activity. Those reporting lentivirus has a luminescent report (Luciferase) or a fluorescent report (GFP), under a minimal CMV promoter (mCMV) that embedded with optimized tandem repeats of E-Box, the c-Myc transcriptional response element (TRE), sequence motif (5′- CACGTG-TGGGA-CACGTG  3′) [Ref 2].

Increased c-Myc levels enhance its ability to bind to E-box sequences in the promoter, leading to the activation of the report. The report’s signal levels reflect the up- or down-regulated c-My clevels. In other words, the levels of c-Myc within a cell are indicative of the activity of the c-Myc signaling pathway.

3. Structure of the Reporting Lentivirus:

The pathway specific reporting Lentivirus use either firefly-luciferase or GFP signal changes to detect the pathway’s activity.   Those reporting lentivirus also contains a constitutively expressed Puromycin antibiotic selection marker, or a GFP fluorescent selection marker, which makes it easier to select the stably transduced signal reporting cells, via Puromycin killing or GFP sorting respectively. See lentivector core scheme below.

The premade, ready-to-use reporter lentivirus provides a much easier tool to monitor the activity of the signaling pathways in virtually any mammalian cell type. It also allows to generate your own reporting cell line in your desired cell type for study or screening of pathway specific gene-knockdown, over-expression, or chemical / drug/protein treatment in the cell based assay.

Click the Product Manual (pdf) for all available products for this pathway reporter.