GATA Pathway

The GATA signaling pathway involves a family of transcription factors known as GATA factors, which play critical roles in the regulation of gene expression during development, differentiation, and various physiological processes. Depending on the cellular context and interacting partners, GATA factors can act as either activators or repressors of gene expression. [Ref1].

2. Lentivirus Detects GATA Pathway:

GenTarget developed a set of reporting lentivirus to monitor GATA signaling pathway activity. Those reporting lentivirus has a luminescent report (Luciferase) or a fluorescent report (GFP), under a minimal CMV promoter (mCMV) that embedded with optimized tandem repeats of pathway specific GATA binding motif as the transcriptional response element (TRE ), sequence motif (5′- GATGATAA-GAAGATAA-GATGATAG  3′) [Ref 2].

Upon the activation of GATA pathway, the GATAs bind to GATA binding motif in the report’s promoter region, leading to increased expression of the reporter protein (luciferase or GFP). This increased expression of the reporter protein can then be quantified using luminescence assays for luciferase or fluorescence assays for GFP. The amount of light produced (luminescence) or GFP fluorescent signal intensity, is proportional to the activity of the GATA pathway.

3. Structure of the Reporting Lentivirus:

The pathway specific reporting Lentivirus use either firefly-luciferase or GFP signal changes to detect the pathway’s activity.   Those reporting lentivirus also contains a constitutively expressed Puromycin antibiotic selection marker, or a GFP fluorescent selection marker, which makes it easier to select the stably transduced signal reporting cells, via Puromycin killing or GFP sorting respectively. See lentivector core scheme below.

The premade, ready-to-use reporter lentivirus provides a much easier tool to monitor the activity of the signaling pathways in virtually any mammalian cell type. It also allows to generate your own reporting cell line in your desired cell type for study or screening of pathway specific gene-knockdown, over-expression, or chemical / drug/protein treatment in the cell based assay.

Click the Product Manual (pdf) for all available products for this pathway reporter.