Progesterone Pathway

The progesterone pathway involves the hormone progesterone, which plays a crucial role in the female reproductive system, particularly in regulating the menstrual cycle, pregnancy, and embryogenesis. It  regulates the transcription of genes involved in reproductive processes and other cellular functions.  [Ref1].

2. Lentivirus Detects Progesterone Pathway:

GenTarget developed a set of reporting lentivirus to monitor Progesterone signaling pathway activity. Those reporting lentivirus has a luminescent report (Luciferase) or a fluorescent report (GFP), under a minimal CMV promoter (mCMV) that embedded with optimized tandem repeats of pathway specific progesterone response elements (PREs) as the transcriptional response element (TRE ), sequence motif (5′- AGAACATGGTGTTCT-GGGACATGGTGTTCT  3′) [Ref 2].

The Progesterone signaling pathway can be activated by natural Progesterone, or synthetic Progestins. Those ligands bind to the progesterone receptors (PR-A and PR-B) in the target cells, which induces a conformational change in the receptor. The activated receptor-ligand complex translocates to the cell nucleus, where binds to specific progesterone response elements (PREs) in this report’s promoter region of this lentivirus, leading to increased expression of the reporter protein (luciferase or GFP).

3. Structure of the Reporting Lentivirus:

The pathway specific reporting Lentivirus use either firefly-luciferase or GFP signal changes to detect the pathway’s activity.   Those reporting lentivirus also contains a constitutively expressed Puromycin antibiotic selection marker, or a GFP fluorescent selection marker, which makes it easier to select the stably transduced signal reporting cells, via Puromycin killing or GFP sorting respectively. See lentivector core scheme below.

The premade, ready-to-use reporter lentivirus provides a much easier tool to monitor the activity of the signaling pathways in virtually any mammalian cell type. It also allows to generate your own reporting cell line in your desired cell type for study or screening of pathway specific gene-knockdown, over-expression, or chemical / drug/protein treatment in the cell based assay.

Click the Product Manual (pdf) for all available products for this pathway reporter.