STAT3 Pathway

Both STAT3 and STAT5 are part of the STAT family and share a common activation mechanism involving cytokine receptors and JAKs. The STAT3 and STAT5 signaling pathways regulate a wide range of biological processes, including cell growth, differentiation, survival, and immune responses [Ref1].

2. Lentivirus Detects STAT3/5 Pathway:

GenTarget developed a set of reporting lentivirus to monitor STAT3/5 signaling pathway activity. Those reporting lentivirus has a luminescent report (Luciferase) or a fluorescent report (GFP), under a minimal CMV promoter (mCMV) that embedded with optimized tandem repeats of STAT3/5 pathway-specific transcriptional response element (TRE / GAS sites ), sequence motif (5′- GTCGACATTTCCCGTAAATCGTCGA-TTCCCGGAA   3′) [Ref 2].

When Cytokines and growth factors bind to their specific receptors on the cell surface (for example, IL6 for STAT3 pathway, and IL-2 for STAT5 pathway), the JAKs is activated, then STAT3 or STAT5 are recruited and phosphorylated by JAKs. Phosphorylated STAT3 or STAT5 binds to the GAS motifs in the promoter regions of this report lentivirus, leading to expression of the report, the Luciferase or GFP.

3. Structure of the Reporting Lentivirus:

The pathway specific reporting Lentivirus use either firefly-luciferase or GFP signal changes to detect the pathway’s activity.   Those reporting lentivirus also contains a constitutively expressed Puromycin antibiotic selection marker, or a GFP fluorescent selection marker, which makes it easier to select the stably transduced signal reporting cells, via Puromycin killing or GFP sorting respectively. See lentivector core scheme below.

The premade, ready-to-use reporter lentivirus provides a much easier tool to monitor the activity of the signaling pathways in virtually any mammalian cell type. It also allows to generate your own reporting cell line in your desired cell type for study or screening of pathway specific gene-knockdown, over-expression, or chemical / drug/protein treatment in the cell based assay.

Click the Product Manual (pdf) for all available products for this pathway reporter.