Description
Pre-made lentivirus expresses human target, AKR1C3 (human aldo-keto reductase family 1 member C3) (alternative name: DD3; DDX; HA1753; HAKRB; HAKRe; hluPGFS; HSD17B5; PGFS). The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID:NM_003739.5
The target is natively expressed under an optional inducible suCMV promoter. This lentivirus can be used for regular constitutive high expression (without need for any induction) or optionally used for inducible expression with tetracycline or Dox induction when the tetracycline repressor protein (TetR) is present.
A GFP-Puromycin fusion dual marker under the RSV promoter allows fluorescence sorting or Puromycin resistance selection of transduced cells. The GFP signal additionally provides convenient, real-time monitoring of the particles’ performance.
See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 108 IFU/ml, concentrated lentivirus provided in PBS solution, premised with Polybrene. (Note: see also the RFP-Blasticidin version of this product, CAT#: LVP1129.).
Cat#: LVP1129-GP-PBS
