Description
Pre-made lentivirus expresses human target, BRAF (B-Raf proto-oncogene, serine/threonine kinase ), Alternative Names: B-raf; B-RAF1; BRAF1; NS7; RAFB1. The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID: NM_004333
The target is natively expressed under an optional inducible suCMV promoter. This lentivirus can be used for regular constitutive high expression (without any induction) or used for inducible expression upon tetracycline or Dox induction when the tetracycline repressor protein (TetR) is present.
A RFP_Blasticidin (Fluorescent-Antibiotic) Fusion dual marker under the RSV promoter allows fluorescence sorting or blasticidin resistance selection of transduced cells. The RFP signal additionally provides convenient, real-time monitoring of the lentivirus’ performance.
Each Lentivirus is validated on lot-by-lot basis, and target expression is guaranteed. See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 108 IFU/ml (provided in 10x of polybrene, 60 ug/ml)
Cat#: LVP1156