Description
Pre-made lentivirus express CRE recombinase under Optional inducible CMV promoter. CRE contains a NLS (nuclear localization signal) at its N-term. This nuclear permeant CRE increases the recombination events in vivo. A Puromycin marker under RSV promoter, allows to select transduced cells for long term expression via Puromycin killing.See Product Manual for details (.pdf)
Cre protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences, named lox sites. CRE lentiviral particles can catalyze the jointing event In Vivo in the presence of lox sites. Purified CRE enzyme can joint individual plasmids containing lox sites.
CRE is constitutively expressed in high level from this product. And optionally, also can be used as tetracycline inducible expression as desired when its repressor is present.
Amount: 200ul/per vial, at 1 x 108 IFU/ml (provided in PBS, premixed with 10x Polybrene, 60 ug/ml)
Cat#: LVP339-PBS
