Description
Pre-made lentivirus express nuclear localized Flp recombinase (Flippase) under suCMV promoter, carrying RFP–Blasticidin fusion dual marker under RSV promoter, allowing to sort or select transduced cells via RFP signal or via blasticidin antibiotic. RFP signal provides a convenient, realtime monitoring the particles performance. See Product Manual (.pdf).
Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism’s DNA under controlled conditions in vivo. It is analogous to Cre-lox recombination but involves the recombination of sequences between short flippase recognition target (FRT) sites by the recombinase flippase (Flp).
The 34bp minimal FRT site sequence has the sequence
5’GAAGTTCCTATTCtctagaaaGtATAGGAACTTC3′
Amount: 200ul/per vial, at 1 x 108 IFU/ml (premixed with 10x polybrene)
Cat#: LVP014
