Description
Lentivirus expresses human CARM1 gene, (Gene_Name: coactivator associated arginine methyltransferase 1 ), with coding sequence 100% matching to CDS region in NCBI ID: NM_199141 (gene_synonym: NAPI-3B; NAPI-IIb; NaPi2b; NPTIIb; PULAM ). see the expression Lentivector scheme below.
The target was expressed under the optional inducible CMV promoter (TetCMV) (click to see more technical details). It constitutively expresses the target without need for any induction.
Yet, optionally, It can be used for the inducible expression when used together with its repressor (TetR). For generating inducible expression cell line, simply apply this target expression lentivirus into TetR ready Cell lines.
It also contains the RFP-Blasticidin fusion dual selection marker, under RSV promoter, allows to sort transduced cells via RFP signal, or select via blasticidin antibiotic killing. RFP signal provides convenient, real-time monitoring of the performance of the particles.
When desired, use the Null-control Lentivirus (CAT#: CMV-Null-RB-PBS) as the negative control, for lentivirus treatment in your cell culture. The Null-Control does not express any targets, but has the same RFP-Blasticidin selection marker.
See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 108 IFU/ml (Concentrated lentivirus provided in PBS solution, premixed with polybrene / 60ug/ml )
Cat#: LVP1777
