Description
Premade Lentivirus expresses human target: CFTR (alternative name: CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1). The sub-cloned codon sequence is identical (100% match) to CDS region in the target, NCBI ID: NM_000492.
The target is natively expressed under a optional inducible suCMV promoter (Note: it can also used as regular constitutive high expression without any inducation required). A GFP-Puromycin fusion dual selection marker under RSV promoter, allows to sort or select transduced cells via RFP signal or via blasticidin antibiotic (Dual markers). GFP signal provides a convenient, realtime monitoring the performance of the particle.
See Product Manual for details (.pdf). (See also the RFP–Blasticidin version of this product, CAT#: LVP518)
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium with 10x Polybrene. (Note: the concentrated lentivirus, CAT#: LVP518-GP-PBS, is available upon request, at price $650 USD/vial -200ul).
Cat#: LVP518-GP
