h FLT3-D835Y (RFP-Bsd), Concentrated Lentivirus

$650.00

Lentivirus expresses human, Flt3-D835Y ( mutant), with RFPBlasticidin dual selection

SKU: LVP1761 Categories: ,

Description

This Pre-made Lentivirus expresses human, Flt3-D835Y ( mutant). Click to see its coding sequence. The expression lentivector’s core structure is listed in the scheme below.

Lentivector map with TetCMV promoter

The target was expressed under the optional inducible CMV promoter (TetCMV) (click to see more technical details). It constitutively expresses the target.  And optionally,  It can be used for the inducible expression when used together with its repressor (TetR). It also contains the RFP-Blasticidin fusion dual selection marker, under RSV promoter, allows to sort  transduced cells via RFP signal, or select via blasticidin antibiotic killing. RFP signal provides convenient, realtime monitoring of the performance of the particles. 

When desired, use the cognate Null-control Lentivirus (CAT#: CMV-Null-RB-PBS) as the negative control, for lentivirus treatment in your cell culture. The Null-Control does not express any targets, but has the same RFP-Blasticidin selection marker.

See Product Manual for details (.pdf).

Amount: 200ul, at 1 x 108 IFU/ml (Concentrated lentivirus premixed with polybrene / 60ug/ml )

Cat#: LVP1761