Description
Lentivirus expresses human target, KRAS: Kirsten rat sarcoma viral oncogene homolog (alternative name: C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RASK2 ). The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID: NM_004985.
The target was expressed with a N-terminal HiBit tag. The HiBit tag is derived from the naturally occurring bioluminescent protein NanoLuc, which emits a bright luminescent signal upon interaction with a specific substrate. When the HiBit tag is fused to a protein of interest, it can be used to detect and quantify the expression of the tagged protein in cells or tissues.
The target was expressed under the optional inducible CMV promoter (TetCMV) (click to see more technical details). It constitutively expresses the target. And optionally, It can be used for the inducible expression when used together with its repressor (TetR). It also contains the RFP-Blasticidin fusion dual selection marker, under RSV promoter, allows to sort or select transduced cells via RFP signal or via blasticidin antibiotic. RFP signal provides convenient, realtime monitoring of the performance of the particles. (see also the GFP-Puro version, CAT#: LVP1639)
When desired, use the cognate Null-control Lentivirus (CAT#: CMV-Null-RB) as the negative control, for lentivirus treatment in your cell culture. The Null-Control does not express any targets, but has the same RFP-Blasticidin selection marker.
See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 108 IFU/ml (Concentrated lentivirus provided in PBS solution, premixed with polybrene / 60ug/ml )
Cat#: LVP1637
