Description
Lentivirus expresses human target: HLA-C gene (Human HLA class I histocompatibility antigen, C alpha chain precursor), (Gene_Synonym: D6S204; HLA-JY3; HLAC; HLC-C; MHC; PSORS1), with amino acid sequence 100% matching to NCBI ID: NP_002108.4. It is expressed with C-terminal HiBit tag. The HiBit tag is derived from the naturally occurring bioluminescent protein NanoLuc, which emits a bright luminescent signal upon interaction with a specific substrate. When the HiBit tag is fused to a protein of interest, it can be used to detect and quantify the expression of the tagged protein in cells or tissues.
The target was expressed under the optional inducible CMV promoter (TetCMV) (click to see more technical details). It constitutively expresses the target. Also optionally, It can be used for the inducible expression when used together with its repressor (TetR). An RFP-Blasticidin fusion dual selection marker under RSV promoter, allows to sort or select transduced cells via RFP signal or via blasticidin antibiotic. RFP signal provides convenient, realtime monitoring of the performance of the particles.
When desired, use the Null-control Lentivirus (CAT#: CMV-Null-RB) as the negative control for lentivirus treatment in your cell culture. The Null-Control does not express any targets, but has the same selection marker expressed.
See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in (premixed with 10x of polybrene (60 ug/ml).
Cat#: LVP1417
