Description
Lentivirus expresses human IL12b gene, (Gene_Name: interleukin 12B ), with coding sequence 100% matching to CDS region in NCBI ID: NM_002187 (gene_synonym: CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF; NKSF2).
The target was expressed under the optional inducible CMV promoter (TetCMV) (click to see more technical details). It constitutively expresses the target. And optionally, It can be used for the inducible expression when used together with its repressor (TetR). It also contains the RFP-Blasticidin fusion dual selection marker, under RSV promoter, allows to sort transduced cells via RFP signal, or select via blasticidin antibiotic killing. RFP signal provides convenient, realtime monitoring of the performance of the particles.
When desired, use the cognate Null-control Lentivirus (CAT#: CMV-Null-RB-PBS) as the negative control, for lentivirus treatment in your cell culture. The Null-Control does not express any targets, but has the same RFP-Blasticidin selection marker.
See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 108 IFU/ml (Concentrated lentivirus provided in PBS solution, premixed with polybrene / 60ug/ml )
Cat#: LVP1759
