Description
Lentivirus expresses human target: MSLN gene (mesothelin), (Gene_Synonym: MPF; SMRP), with coding sequence 100% matching to CDS region in NCBI ID: NM_005823. It contains a RFP–Blasticidin dual selection marker.
The target was expressed under the optional inducible CMV promoter (TetCMV) (click to see more technical details). It constitutively expresses the target. Also optionally, It can be used for the inducible expression when used together with its repressor (TetR). An RFP-Blasticidin fusion dual selection marker, under RSV promoter, allows to sort or select transduced cells via RFP signal or via blasticidin antibiotic. RFP signal provides convenient, realtime monitoring of the performance of the particles.
When desired, use the Null-control Lentivirus (CAT#: CMV-Null-RB) as the negative control for lentivirus treatment in your cell culture. The Null-Control does not express any targets, but has the same RFP-Blasticidin selection marker.See Product Manual for details (.pdf).
This is the concentrated lentivirus, best suited for hard-to-transduced cell types, or in vivo application.
Amount: 200ul, at 1 x 108 IFU/ml in PBS (premixed with Polybrene).
Cat#: LVP1378-PBS
