Human RPMI 8226 (Luciferase & RFP) Stable Cells

$2,500.00

Human RPMI 8226 (Luciferase & RFP) dual report Stable Cell line

SKU: SC060-LR Categories: ,

Description

The human RPMI 8226 cell line ( a B Lymphocyte with surface antigen profile: HLA Aw19, B15, B37, Cw2; CD19 -; CD20 -; CD28 +; CD38 +; CD49e +) was originated from a human peripheral blood with multiple myeloma from 61 years male.

This cell line was transformed from RPMI 8226, having the firefly Luciferase and RFP fluorescent report constitutively co-expressed under EF1a promoter as two separate proteins (not as fusion), mediated via 2A element. The Puromycin resistance marker was expressed under Rsv promoter. The following express cassette was integrated into cell line’s genome. 

Lentivector scheme express Luc and RFP

Cells demonstrate strong RFP fluorescent signal, and luciferase assay signal via Luc assay (Note: It is the pooled stable cell population, not originated from a single cell colony. Thus you will observe the uneven RFP signal intensity amount cells).

Sold at:  1 vial x (2 x 106 cells)/vial, see details in Product Manual.

Cat# SC060-LR