Human NCI-H1650 / Luciferase & RFP Stable Cell Line

$4,000.00

human NCI-H1650 cell line co-express Luciferase and RFP dual reporter.

Description

Human NCI-H1650 is a human cancer cell line derived from a patient with non-small cell lung cancer (NSCLC), from 27-year-old male with bronchoalveolar carcinoma. It is an adherent cell line with epithelial-like morphology. NCI-H1650 is extensively used in cancer research to understand the biological mechanisms and pathways involved in lung adenocarcinoma. 

This Luciferase & RFP / human NCI-H1650 cell line was transformed via Lentivirus transduction. It has the Luciferase and RFP was constitutively co-expressed under the same enhanced CMV promoter (suCMV). The Puromycin resistance marker was expressed under Rsv promoter. The following express cassette was integrated into cell-line’s genome. 

Luc and GFP co-expression map scheme

Each cell demonstrates a strong RFP fluorescent signal (see sample image below). Its chem-luminescence signal was verified via luciferase assay using the D-luciferin substrate, which can be detected, in vitro and in vivo.

Sold at:  1 vial x (2 x 106 cells)/vial, see details in Product Manual.

Cat# SC098-LR