Description
Lentivirus express the “LoxP-GFP-stop-LoxP-RFP-Stop” cassette under the enhanced EF1a promoter. It is used to monitor or validate the efficiency of the CRE recombination reaction. see Product Manual for details.
EF1a promoter is active in most or all tissues. Although EF1a promoter is not as strong as viral promoters like CMV, it tends not to get silenced (like CMV does). The lentivirus also contains a puromycin antibiotic selection marker under Rsv promoter.
Amount: 200 ul at 1 x 108 IFU/ml (concentrated lentivirus provided in PBS solution, premixed with 10x Polybrene / 60ug/ml)
CAT#: LVP1332-Puro-PBS