Description
Lentivirus express luciferase II gene under the enhanced EF1a promoter. A GFP marker was co-expressed under the same promoter. Luciferase and GFP were bicistronically expressed as individual protein mediated by a 2A element. A Blasticidin antibiotic marker was expressed under RSV promoter, allowing to sort or select transduced cells for long-term expression via GFP signal or via blasticidin antibiotic (Dual selection). GFP signal provides a convenient, real-time monitoring the particle’s performance.
Particles was concentrated and buffer exchanged into PBS. See Product Manual for details (.pdf).
Amount: 200ul/per vial, at 1 x 108 IFU/ml in PBS.
Cat#: LVP436-PBS
