Description
Pre-packaged rAAV in Serotype-DJ, constitutively express the human codon-optimized Green Fluorescent Protein (GFP) (no tag) under a modified EF1a Promoter. This engineed EF1a promoter with smaller size, best fit AAV packaging capacity, for high expression levels in a wide range of mammalian cells, less tissue-specific in promoter strength and No promoter-silencing effect as that for CMV promoter in some cell types.
The following scheme showed the AAV vector’s core structure. See Product Manual for details.
AAV Serotype-DJ is a synthetic serotype made from different serotypes using DNA shuffling. AAV-DJ serotype has the enhanced transduction efficiency in vitro and in vivo compared to wild-type serotypes with Broad Cell Tropism, great transduction rate in CNS, Liver, Kidney, Fibroblasts, muscle. To determine the serotype that best suits your desired cell types, you can use the AAV-GFP (CMV) Serotype Screening Kit.
Titer: ~ 1×10^12 GC/mL
Size: 100ul/vial, in stock, ready to ship in overnight Dry-ice package.
Storage Buffer: PBS
CAT#: A1.14